Open AccessCharacterization of the gltF gene product of Escherichia coli
Author(s) -
Grassl Guntram,
Bufe Bernd,
Müller Birgit,
Rösel Marc,
Kleiner Diethelm
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08711.x
Subject(s) - periplasmic space , escherichia coli , operon , gene product , structural gene , mutant , biochemistry , gene , biology , catabolism , amino acid , signal peptide , translation (biology) , peptide sequence , chemistry , microbiology and biotechnology , gene expression , enzyme , messenger rna
The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits ( gltB and gltD ) and gltF , whose product was previously suggested to have regulatory functions. The A/T‐rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF . The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A gltF ::Km R insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.