Open AccessAn investigation into the electro‐physical characteristics of microbial cells during the metabolism of toxic compounds under conditions of limited O 2 availability
Author(s) -
Ignatov Oleg V.,
Khorkitaliya A.,
Shchyogolev Sergei Yu.,
Singirtsev Igor N.,
Bunin Victor D.,
Tumaikina Yuliya A.,
Ignatov Vladimir V.
Publication year - 1999
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1999.tb08685.x
Subject(s) - metabolism , acrylamide , chemistry , substrate (aquarium) , biophysics , enzyme , microbial metabolism , biochemistry , bacteria , biology , organic chemistry , polymer , ecology , genetics , copolymer
The purpose of the work reported here was to experimentally clarify the interconnection between changes in the electro‐physical characteristics of microbial suspensions and processes of the metabolism of certain toxic compounds (acrylamide and p ‐nitrophenol (PNP)) in cells containing enzyme systems of the initial metabolism of these compounds. In this work, we used cells of two strains, Acinetobacter calcoaceticum A‐122 and Brevibacterium sp. 13PA, which are capable of utilising PNP and acrylamide, respectively, as the sole source of carbon. Suspensions of these cells exhibited appreciable decreases in the magnitude of the electro‐optical signal when the microbial metabolism of PNP and acrylamide was inhibited under conditions of limited O 2 supply. This attests to a relationship between the electro‐physical characteristics of microbial suspensions and cellular metabolic reactions and also to the negligibly small effect that the non‐specific interaction of substrate with the cells has on the suspensions’ electro‐optical properties.