Open AccessCloning of a novel gene yrbB , encoding a protein located in the spore integument of Bacillus subtilis
Author(s) -
Takamatsu Hiromu,
Hiraoka Takeyuki,
Kodama Takeko,
Koide Hidekatsu,
Kozuka Satoshi,
Tochikubo Kunio,
Watabe Kazuhito
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13913.x
Subject(s) - bacillus subtilis , cloning (programming) , gene , biology , spore , molecular cloning , integument , microbiology and biotechnology , genetics , bacteria , anatomy , gene expression , computer science , programming language
A DNA fragment (2.7 kbp) containing three deduced open reading frames, orf1, orf2 and orf3 (partial sequence), was isolated from the genomic library of Bacillus subtilis using an antiserum raised against spore integument, and was sequenced. orf2 was 519 nucleotides long and encoded a protein of 172 amino acids with a predicted molecular size of 19552, corresponding to the protein which reacted with the antiserum. Immunoelectron microscopic observation indicated that YrbB, the product of orf2 , was located within the spore integument, mainly in the cortex layer with a part in the inner region of the coat layer.