Open AccessActivation of a consensus FNR‐dependent promoter by DNR of Pseudomonas aeruginosa in response to nitrite
Author(s) -
Hasegawa Norio,
Arai Hiroyuki,
Igarashi Yasuo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13892.x
Subject(s) - pseudomonas aeruginosa , mutant , nitrite , microbiology and biotechnology , promoter , biology , chemistry , biochemistry , nitrate , gene , gene expression , bacteria , genetics , ecology
Expression of the enzymes for anaerobic nitrate respiration of Pseudomonas aeruginosa requires two CRP/FNR‐related transcriptional regulators, ANR and DNR. Activity of the consensus CRP‐ or FNR‐dependent promoter in the anr and dnr deficient mutants was investigated. The CRP‐dependent promoter was active in the mutant strains. Both regulators could activate the promoter with a consensus FNR‐binding motif. DNR‐dependent activation was nitrite‐dependent, whereas activation by ANR was not, suggesting that only DNR is involved in sensing nitrogen oxides.