Open AccessInternal size variations in Tn 1546 ‐like elements due to the presence of IS 1216V
Author(s) -
Jensen Lars Bog?
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13339.x
Subject(s) - gene , sequence (biology) , insertion sequence , biology , genetics , encoding (memory) , gene duplication , transposable element , computational biology , genome , neuroscience
In this study, internal size variations in the VanA gene cluster Tn 1546 , encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn 1546 , revealed that these variations were due to the presence of the IS sequence, IS 1216V . This IS sequence has previously been found integrated in Tn 1546 . Integration of the IS 1216V element created both deletions and a duplication in a non‐essential region of Tn 1546 . In several isolates, the entire vanY gene was deleted, proving that this gene is non‐essential for vancomycin resistance.