Open AccessCharacterization of three capsular polysaccharides produced by Burkholderia pseudomallei
Author(s) -
Kawahara Kazuyoshi,
Dejsirilert Surang,
Ezaki Takayuki
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13330.x
Subject(s) - polysaccharide , rhamnose , mannose , uronic acid , bacteria , galactose , burkholderia pseudomallei , chemistry , burkholderia , biochemistry , monosaccharide , glycerol , microbiology and biotechnology , chemical structure , stereochemistry , biology , organic chemistry , genetics
Three kinds of capsular polysaccharide (CP) were found to be produced by Burkholderia pseudomallei . When the bacterium was grown with the medium without glycerol, CP‐1a and CP‐1b were produced. CP‐1a was mainly 1,4‐linked glucan and CP‐1b was identified as a polymer composed of galactose and 3‐deoxy‐ d ‐ manno ‐octulosonic acid, whose chemical structure was recently reported by other laboratories. When the bacterium was grown with the medium containing 5% glycerol, CP‐2 was synthesized. CP‐2 contained galactose, rhamnose, mannose, glucose and a uronic acid in a ratio of approximately 3:1:0.3:1:1. Methylation analysis of the purified polysaccharides demonstrated that the two acidic polysaccharides, CP‐1b and CP‐2, shared no common structure, indicating that CP‐2 was an acidic capsular polysaccharide whose chemical characters were not reported previously.