Open AccessConstruction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other Gram‐negative bacteria
Author(s) -
Rist Michael,
Kertesz Michael A
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13315.x
Subject(s) - plasmid , promoter , cloning (programming) , biology , carbenicillin , reporter gene , pseudomonas aeruginosa , bacteria , multiple cloning site , cloning vector , gene , genetics , molecular cloning , microbiology and biotechnology , recombinant dna , expression vector , gene expression , computer science , programming language
We report the construction of two broad host range promoter‐probe plasmid vectors for rapid analysis of promoters in Gram‐negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facilitate directional cloning of putative promoter elements. The vectors allow rapid plate‐based screening for promoter activities, using β‐galactosidase as the reporter enzyme. In the absence of an inserted promoter fragment, they display very low background activity, making them a useful tool for analysis of low expression level promoters.