Open AccessHigh levels of transcription factor RpoS (σ S ) in mviA mutants negatively affect 1,2‐propanediol‐dependent transcription of the cob/pdu regulon of Salmonella typhimurium LT2
Author(s) -
Rondon Michelle R,
EscalanteSemerena Jorge C
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13311.x
Subject(s) - regulon , rpos , mutant , propanediol , transcription (linguistics) , propionate , biology , transcription factor , salmonella , genetics , gene , microbiology and biotechnology , gene expression , chemistry , biochemistry , promoter , bacteria , organic chemistry , linguistics , philosophy
Expression of the cobalamin biosynthetic ( cob ) and 1,2‐propanediol utilization ( cob/pdu ) regulon of Salmonella typhimurium LT2 is controlled at the transcriptional level by global and specific regulatory proteins. In this paper we show that mutations in the mviA gene negatively affect cob/pdu transcription in response to 1,2‐propanediol in the environment. The effects of mviA mutations were consistent with its role in the regulation of RpoS levels in the cell. Null mutations in rpoS eliminated the negative effect of mviA mutations on cob/pdu transcription, and restored growth on succinate, propionate and 1,2‐propanediol. In addition, mviA mutants were deficient in the utilization of succinate, propionate and 1,2‐propanediol as carbon and energy sources.