Open AccessProduction of novel polyhydroxyalkanoates by Pseudomonas stutzeri 1317 from glucose and soybean oil
Author(s) -
He Wennan,
Tian Weidong,
Zhang Guang,
Chen GuoQiang,
Zhang Zengming
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13297.x
Subject(s) - polyhydroxyalkanoates , pseudomonas stutzeri , soybean oil , monomer , chemistry , food science , organic chemistry , nuclear chemistry , polymer , bacteria , biology , genetics
A strain of Pseudomonas stutzeri coded 1317 isolated from oil‐contaminated soil was found to grow well in glucose and soybean oil as a sole carbon source, respectively. Polyhydroxyalkanoates containing medium chain length monomers of hydroxyalkanoates ranging from C6 to C14 were synthesized up to 52% of cell dry weight of P. stutzri 1317 grown on glucose mineral media. In mineral media containing 10 g l −1 soybean oil, P. stutzeri 1317 produced up to 63% of polyhydroxyalkanoates containing mainly a novel monomer of 3,6‐epoxy‐7‐nonene‐1,9 dioic acids making up to 63% of the polyhydroxyalkanoates, together with minor monomers of C8 and C10. 1 H‐ and 13 C‐nuclear magnetic resonance spectroscopy and electron impact gas chromatography‐mass spectroscopy and chemical ionization gas chromatography‐mass spectroscopy were used to study the structures of polyhydroxyalkanoates synthesized by P. stutzeri 1317.