Open AccessPeptone changes the timing and accumulation of morphological mutants of the Quorn ? myco‐protein fungus Fusarium graminearum A3/5 in glucose‐limited chemostat cultures
Author(s) -
Wiebe Marilyn G,
Robson Geoffrey D,
Trinci Anthony P.J
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13294.x
Subject(s) - chemostat , mutant , biology , fermentation , population , fungus , fusarium , dilution , biochemistry , microbiology and biotechnology , strain (injury) , fungi imperfecti , food science , botany , bacteria , genetics , gene , demography , physics , sociology , thermodynamics , anatomy
Fusarium graminearum A3/5 was grown at pH 5.8 and a dilution rate of 0.19 h −1 in glucose‐limited chemostat culture supplemented with mycological peptone for 528 h. Although the population changed (i.e. displayed periodic selection) at the same rate in supplemented (132±23 h) and unsupplemented cultures (124±12 h), highly branched mutants were detected much earlier in the former cultures than in the latter, although they failed to displace the sparsely branched parental population. It was demonstrated that the selective advantage (compared to A3/5) of some mutants isolated from unsupplemented glucose‐limited culture could be eliminated by addition of peptone to the medium, but that a general growth rate mutant retained its advantage in the peptone‐supplemented medium. These results could be used to improve the cost‐efficiency of the fermentation.