Open AccessPutative dengue virus receptors from mosquito cells
Author(s) -
Lourdes Muñoz Marı́a,
Cisneros Alejandro,
Cruz Jorge,
Das Pradeep,
Tovar Rosalinda,
Ortega Arturo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13281.x
Subject(s) - dengue virus , dengue fever , virology , aedes albopictus , biology , antibody dependent enhancement , polyclonal antibodies , receptor , virus , antibody , aedes aegypti , genetics , botany , larva
Dengue viruses are arthropod‐borne, single‐stranded RNA viruses. Aëdes aegypti and Aëdes albopictus are the principal vectors. In order to understand the molecular basis of dengue virus infections we explored the biochemical identity of dengue‐2 (DEN‐2) virus receptors in the Aëdes albopictus ‐derived cell line C6/36. We show here that DEN‐2 interacts with two major polypeptides of 80 and 67 kDa. Polyclonal anti‐C6/36 membrane antibodies block DEN‐2 binding to intact C6/36 monolayers as well as to membrane extracts. Our results strongly suggest that the identified polypeptides are part of the DEN‐2 receptors.