Open AccessDetection of Helminthosporium solani from soil and plant tissue with species‐specific PCR primers
Author(s) -
Olivier Claudia,
Loria Rosemary
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13279.x
Subject(s) - biology , primer (cosmetics) , internal transcribed spacer , polymerase chain reaction , ribosomal dna , rhizoctonia solani , nested polymerase chain reaction , nuclear dna , fungus , dna , inoculation , pathogenic fungus , spore , microbiology and biotechnology , botany , ribosomal rna , horticulture , genetics , gene , chemistry , phylogenetics , mitochondrial dna , organic chemistry
Two PCR primer pairs specific for Helminthosporium solani , which causes silver scurf on potato tubers, were designed from nucleotide sequences of the nuclear ribosomal internal transcribed spacer regions of H. solani . Both primer pairs amplified a single product with DNA from 48 North American and European isolates of H. solani , but not with DNA from 42 other fungi. Primers also amplified a single product with DNA extracted from silver scurf lesions on potato tubers and other plant tissue inoculated with spores of H. solani . Detection of the fungus in infested soil was only possible with nested PCR and after processing soil with a bead beater. Specific amplification of H. solani DNA can be used to study the saprophytic and pathogenic activity of this fungus in soil and plant tissue.