Open AccessAnalysis of the regulatory region of the lysC gene of Escherichia coli
Author(s) -
Liao HuiHua,
Hseu TzongHsiung
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13251.x
Subject(s) - promoter , transcription (linguistics) , gene , genetics , biology , plasmid , regulatory sequence , fusion gene , transcription factor , microbiology and biotechnology , gene expression , philosophy , linguistics
A lysC–lac′ Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream the transcription start site of promoter P1. Both promoters are regulated by lysine.