Open AccessThe RAG3 gene of Kluyveromyces lactis is involved in the transcriptional regulation of genes coding for enzymes implicated in pyruvate utilization and genes of the biosynthesis of thiamine pyrophosphate
Author(s) -
Tizzani Lorenza,
Meacock Peter,
Frontali Laura,
WésolowskiLouvel Micheline
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13250.x
Subject(s) - pyruvate decarboxylase , kluyveromyces lactis , gene , biology , pyruvate dehydrogenase complex , regulator gene , kluyveromyces , biochemistry , pyruvate dehydrogenase phosphatase , regulation of gene expression , saccharomyces cerevisiae , enzyme , alcohol dehydrogenase
The RAG3 gene of Kluyveromyces lactis , a homolog of PDC2 of Saccharomyces cerevisiae , is known to be a regulator of the pyruvate decarboxylase gene KlPDC1 . We have identified new target genes for Rag3p. The RAG3 gene product was found to be required for the transcription of two genes of the biosynthetic pathway of thiamine (a cofactor of pyruvate decarboxylase). Conversely, the RAG3 gene product partially repressed the expression of the pyruvate dehydrogenase gene KlPDA1 . Therefore, RAG3 may act as a general regulator in the balance of the two alternative pathways of pyruvate metabolism in yeast.