Open AccessNormal induction of the SOS response in Bacillus subtilis is prevented by the mutant repressor from phage φ105 cts 23
Author(s) -
Rubinstein Clara P,
Guerchicoff Alejandra,
SanchezRivas Carmen
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13245.x
Subject(s) - prophage , repressor , repressor lexa , mutant , bacillus subtilis , biology , sos response , lysogenic cycle , nalidixic acid , microbiology and biotechnology , reversion , mutation , derepression , suppressor mutation , genetics , psychological repression , bacteriophage , gene , escherichia coli , phenotype , gene expression , bacteria
The presence of the Φ105 cts 23 mutant prophage in Bacillus subtilis induces a series of pleiotropic effects that could be ascribed to an anti‐SOS activity. In order to circumvent the phage function responsible for this phenomenon, the cts 23 mutant repressor was cloned and sequenced. The isolated repressor reduced the survival capacity of the host cells after mitomycin C or nalidixic acid treatments and lowered the spontaneous reversion frequency. When SOS induction kinetics were studied, low or null induction of the damage‐inducible din 22:: Lac Z fusion was observed. In contrast, the presence of the wild‐type prophage amplified the SOS response. Sequencing of the mutant repressor revealed that the cts 23 mutation is a T→C transition affecting the 5′ closest codon to one of the two reported DNA binding domains.