Open AccessDemonstration of the third antigenically distinct outer membrane lipoprotein (OmlA) in Actinobacillus pleuropneumoniae serotype 7
Author(s) -
Ito Hiroya,
Osaki Makoto,
Uchida Ikuo,
Ohya Tatsuo,
Sekizaki Tsutomu
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13243.x
Subject(s) - serotype , actinobacillus pleuropneumoniae , bacterial outer membrane , biology , antiserum , microbiology and biotechnology , western blot , strain (injury) , virology , gene , peptide sequence , recombinant dna , plasmid , antibody , genetics , escherichia coli , anatomy
The gene encoding an outer membrane lipoprotein (OmlA) of Actinobacillus pleuropneumoniae strain WF83 (serotype 7 reference strain), designated omlA 7 , was sequenced. The amino acid sequence of OmlA 7 showed 64.5 and 71.6% identity to that of OmlA from serotypes 1 (OmlA 1 ) and 5 (OmlA 5 ), respectively. The first 134 amino acids of OmlA 7 were identical to those of OmlA 5 . A Southern blot analysis revealed the presence of a gene highly homologous to the omlA 7 in the reference strains of serotypes 3, 4, 6, and 7. A Western blot analysis using a specific antiserum against a recombinant OmlA 7 detected expression of the homologous proteins in the serotypes 4, 6, and 7 reference strains and a serotype 3 field strain, but not in a serotype 3 reference strain. The data demonstrate the third antigenically distinct OmlA is expressed in A. pleuropneumoniae .