Open AccessMolecular characterization of Salmonella enterica subsp. enterica serovar Typhimurium DT009 isolates: differentiation of the live vaccine strain Zoosaloral from field isolates
Author(s) -
Frech Gabriele,
WeideBotjes Martina,
Nußbeck Erika,
Rabsch Wolfgang,
Schwarz Stefan
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13237.x
Subject(s) - salmonella enterica , serotype , biology , microbiology and biotechnology , plasmid , typing , salmonella , phage typing , strain (injury) , pulsed field gel electrophoresis , virulence , virology , enterobacteriaceae , genetics , escherichia coli , bacteria , genotype , gene , anatomy
A total of 25 epidemiologically unrelated Salmonella enterica subsp. enterica ( S .) serovar Typhimurium DT009 isolates from various human and animal sources, the original S . Typhimurium DT009 Zoosaloral live vaccine strain and two Zoosaloral strains reisolated from vaccinated chickens were investigated by various molecular typing methods (I) to determine the most suitable method or combination of methods for the differentiation of DT009 field isolates and (II) to investigate which molecular methods are suitable to differentiate the Zoosaloral live vaccine strain from field isolates of the same phage type. Based on the results of plasmid profile analysis, IS 200 typing and macrorestriction analysis with Xba I, Spe I and Bln I, the 28 S . Typhimurium DT009 isolates were assigned to 16 different genomic groups, one of which was exclusively represented by the original and the reisolated Zoosaloral strains. IS 200 typing was the most discriminatory single method for the differentiation of the DT009 isolates followed by plasmid profile analysis and Bln I‐macrorestriction analysis. The Zoosaloral vaccine strain differed from the DT009 field isolates by its unique Hin dIII‐fragment pattern of the virulence plasmid and by its unique Spe I‐macrorestriction pattern.