Open AccessProduction of ligninolytic enzymes by Pleurotus sp. and Dichomitus squalens in soil and lignocellulose substrate as influenced by soil microorganisms
Author(s) -
Lang Elke,
Nerud Frantisek,
Zadrazil Frantisek
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13234.x
Subject(s) - straw , pleurotus , microorganism , manganese peroxidase , chemistry , laccase , food science , substrate (aquarium) , environmental remediation , soil water , soil microbiology , biology , enzyme , environmental chemistry , agronomy , biochemistry , contamination , mushroom , bacteria , ecology , genetics
The activities of the extracellular enzymes laccase and manganese peroxidase (MnP) of the white rot fungi Pleurotus sp. and Dichomitus squalens were measured in the straw substrate and soil layers of solid state cultures. Cultures with sterile soil were compared to cultures with nonsterile soil. For both organisms, enzyme activities in sterile soil were approximately the same as in straw when calculated to the base of the liquid phases (water content) in the straw or soil material. The growth rate and enzyme activities of Pleurotus sp. were not significantly influenced by the presence of soil microorganisms. In contrast, D. squalens did not penetrate the nonsterile soil, and no enzyme activities could be detected in nonsterile soil. Laccase and MnP activities in the straw declined to zero. From this point of view, highly competitive strains like Pleurotus sp. seem to be more suitable for the application in soil remediation than weak competitors ( D. squalens ) which are not able to maintain appropriate levels of enzyme activities in nonsterile soil.