Open AccessRapid identification of human intestinal bifidobacteria by 16S rRNA‐targeted species‐ and group‐specific primers
Author(s) -
Matsuki Takahiro,
Watanabe Koichi,
Tanaka Ryuichiro,
Oyaizu Hiroshi
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13216.x
Subject(s) - bifidobacterium bifidum , biology , bifidobacterium longum , actinomycetaceae , bifidobacterium , 16s ribosomal rna , microbiology and biotechnology , bifidobacterium breve , group b , bacteria , human gastrointestinal tract , group a , ribosomal rna , gastrointestinal tract , actinomycetales , genetics , streptomyces , gene , biochemistry , lactobacillus , medicine
On the basis of 16S rRNA sequences, species‐ and group‐specific primers for Bifidobacterium adolescentis, B. angulatum, B. bifidum, B. breve , the B. catenulatum group ( B. catenulatum and B. pseudocatenulatum ), and the B. longum group ( B. longum and B. infantis ), which are species commonly found in human intestinal tracts, were developed. The specificity of these primers was confirmed through the use of DNA extracted from 46 strains of 31 Bifidobacterium species, as well as 14 non‐bifidobacterial species that are the predominant bacteria in the human intestinal tract. The present species‐specific primers were applied to the identification of 43 isolated strains, consisting of six strains of B. adolescentis , eight of the B. catenulatum group, seven of B. bifidum , nine of B. breve , and 13 of the B. longum group.