Open AccessThe GroEL‐like protein from Campylobacter rectus : immunological characterization and interleukin‐6 and ‐8 induction in human gingival fibroblast
Author(s) -
Hinode Daisuke,
Yoshioka Masami,
Tanabe ShinIchi,
Miki Osamu,
Masuda Kaname,
Nakamura Ryo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13199.x
Subject(s) - groel , microbiology and biotechnology , biology , affinity chromatography , monoclonal antibody , fibroblast , biochemistry , antibody , escherichia coli , in vitro , immunology , enzyme , gene
The native GroEL‐like protein was purified from Campylobacter rectus , a putative periodontal pathogen, by affinity chromatography on ATP‐agarose followed by high performance liquid chromatography on Superose 6. The purified 64‐kDa protein (denatured form of GroEL‐like protein) was immunoreactive by SDS‐PAGE and Western immunoblotting with the monoclonal antibody directed against heat shock protein 60 of human origin. The native GroEL‐like protein stimulated both interleukin‐6 (IL‐6) and IL‐8 secretion by a confluent monolayer of human gingival fibroblast in their culture supernatant. During the 22‐h incubation, the amounts of IL‐6 and IL‐8 were increased by 5.4‐ and 3.5‐fold, respectively. These data suggested that the GroEL‐like protein might be considered to be a virulence factor of C. rectus in periodontal disease.