Open AccessUnique morphogenesis in Saccharomyces cerevisiae strain GS1731
Author(s) -
Singh Vineet K,
McCracken Derek A,
Nadakavukaren Mathew J,
Jayaswal R.K
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13188.x
Subject(s) - multinucleate , dapi , cytokinesis , saccharomyces cerevisiae , biology , microbiology and biotechnology , cytoplasm , morphogenesis , strain (injury) , cell division , staining , mitosis , cell growth , cell , yeast , genetics , anatomy , gene
During the lag and early exponential phase of growth, 50–60% of budded cells of Saccharomyces cerevisiae strain GS1731 were multiply budded. During subsequent culture growth, the frequency of multiply budded cells decreased until by stationary phase multiply budded cells were rare. Data from renewed growth of a culture after hydroxyurea treatment indicated that GS1731 mother cells could assemble up to three pre‐bud sites and begin bud growth and development in each. Light and scanning electron microscopy showed two or three very small buds emerging simultaneously on a mother cell and either reaching full size at the same time or enlarging sequentially. Immunofluorescence studies revealed that these multiply budded cells had multiple bundles of cytoplasmic microtubules. DAPI staining of nuclei revealed that some of the unbudded mother cells were multinucleate and completed cytokinesis giving rise to normal daughter cells.