Open AccessCo‐detection of Helicobacter pylori and of its resistance to clarithromycin by PCR
Author(s) -
Sevin E,
Lamarque D,
Delchier J.C,
Soussy C.J,
Tankovic J
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13172.x
Subject(s) - clarithromycin , helicobacter pylori , restriction fragment length polymorphism , 23s ribosomal rna , polymerase chain reaction , biology , restriction enzyme , microbiology and biotechnology , genetics , gene , ribosome , rna
Our aim was to develop a rapid molecular test based on polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) and making it possible to detect Helicobacter pylori directly from gastric biopsy samples, and to test its susceptibility to clarithromycin. A 629‐bp fragment of the 23S rRNA gene of H. pylori was amplified by PCR and the mutations responsible for clarithromycin resistance were detected with Bsa I and Bbs I restriction endonucleases. Thirty‐five gastric samples were tested in parallel by standard microbiologic methods (culture and clarithromycin susceptibility testing with E‐test strips) and by PCR‐RFLP. The 10 culture‐negative samples were also PCR‐negative. Sixteen out of the 25 culture‐positive samples (64%) were PCR‐positive. RFLP analysis could be done in 12 cases and the results were in agreement with those of the E‐test: susceptibility in five cases, resistance in seven (six A2144G mutations and one A2143G mutation).