Open AccessComparison of ARDRA and rec A‐RFLP analysis for genomic species identification of Acinetobacter spp.
Author(s) -
Jawad A.,
Snelling A.M.,
Heritage J.,
Hawkey P.M.
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13170.x
Subject(s) - acinetobacter , amplified ribosomal dna restriction analysis , biology , restriction fragment length polymorphism , genomic dna , genetics , microbiology and biotechnology , ribosomal dna , 16s ribosomal rna , restriction map , ribosomal rna , dna , polymerase chain reaction , phylogenetic tree , bacteria , gene , plasmid
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR‐based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well‐characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of rec A PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the rec A gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of rec A RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined.