Open AccessDevelopment of an integrative DNA transformation system for the yeast Candida utilis
Author(s) -
Rodrı́guez Luis,
Chávez Francisco P.,
Basabe Liliana,
Rivero Tanilo,
Delgado Julio M.
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13166.x
Subject(s) - ura3 , auxotrophy , transformation (genetics) , biology , homologous recombination , yeast , electroporation , dna , genetics , mutagenesis , homologous chromosome , gene , locus (genetics) , biochemistry , saccharomyces cerevisiae , mutation , mutant
We report here the development of an auxotrophic transformation system for the food yeast Candida utilis . To facilitate molecular studies in Candida utilis , we isolated auxotrophic strains for uracil biosynthesis by the combination of NTG‐mutagenesis and 5‐fluorotic acid (FOA) selection. The ura − mutation could be functionally complemented by the homologous URA3 gene. We used both, LiAc and electroporation methods to direct insertions at the ura3 locus through homologous recombination.