Open AccessCharacterization of enteroinvasive Escherichia coli and Shigella strains by RAPD analysis
Author(s) -
Bando Silvia Yumi,
Valle Gloria R.F,
Martinez Marina B,
Trabulsi Luiz R,
MoreiraFilho Carlos A
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13141.x
Subject(s) - shigella , rapd , biology , dendrogram , serotype , jaccard index , microbiology and biotechnology , escherichia coli , genetics , gene , genetic diversity , population , mathematics , statistics , cluster analysis , demography , sociology
Genetic variation of 33 enteroinvasive Escherichia coli (EIEC), 12 non‐EIEC and 39 Shigella strains (representing the 4 species of this genus) was analyzed using the random amplified polymorphic DNA (RAPD) technique. Reproducible polymorphisms were generated and the combined data allowed us to construct a dendrogram using Jaccard's distance. Two main groups were obtained: one for Shigella and the other for EIEC and non‐EIEC strains. The first group contained four clusters, one for each Shigella species. The second group contained one cluster for EIEC and another for non‐EIEC strains. The main clusters encompassed many small clusters corresponding to different serotypes. It was possible to characterize each one of the 84 strains under study as well as the boundaries among Shigella species and between this genus and EIEC strains.