Open AccessCharacterization of Vibrio vulnificus isolated from cockles ( Anadara granosa ): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis
Author(s) -
Radu Son,
Elhadi Nasreldin,
Hassan Zaiton,
Rusul Gulam,
Lihan Samuel,
Fifadara Nimita,
Yuherman ,
Purwati Endang
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13138.x
Subject(s) - vibrio vulnificus , biology , microbiology and biotechnology , plasmid , rapd , antimicrobial , antibiotic resistance , vibrio , antibiotics , dna , bacteria , genetics , population , genetic diversity , demography , sociology
Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles ( Anadara granosa ). Thirty‐six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid DNA bands ranging in size from 1.4 to 9.7 MDa. Thirty‐one (83.3%) were found to be resistant to one or more of the antimicrobial agents tested, however no specific correlation between antimicrobial resistance patterns and a single biotype was found. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers produced polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotype 1 and biotype 2 of the V . vulnificus strains investigated. RAPD identity across biotypes was also observed among Vibrio vulnificus strains.