Open AccessSoluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth
Author(s) -
Arikawa Yukihiko,
Enomoto Keiichiro,
Muratsubaki Haruhiro,
Okazaki Mitsuo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13134.x
Subject(s) - saccharomyces cerevisiae , fumarate reductase , biochemistry , biology , anaerobic exercise , isozyme , reductase , tonicity , gene , enzyme , escherichia coli , physiology
In Saccharomyces cerevisiae , the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneous disruption of the FRDS and OSM1 genes resulted in the inability of the yeasts to grow anaerobically on glucose as a carbon source, and disruption of the OSM1 gene caused poor growth under anaerobic conditions. However, the disruption of both the FRDS and/or OSM1 genes had no effect on aerobic growth or growth under hypertonic conditions. These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions.