Open AccessRestriction fragment length polymorphism species‐specific patterns in the identification of white truffles
Author(s) -
Bertini Luana,
Potenza Lucia,
Zambonelli Alessandra,
Amicucci Antonella,
Stocchi Vilberto
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13115.x
Subject(s) - biology , truffle , restriction fragment length polymorphism , restriction enzyme , restriction site , genetics , polymerase chain reaction , ribosomal dna , terminal restriction fragment length polymorphism , restriction fragment , specific identification , microbiology and biotechnology , dna , botany , gene , phylogenetic tree
A molecular method for the identification of ectomycorrhizae belonging to five species of white truffle is described. The polymerase chain reaction (PCR) and universal primers were used to amplify internal transcribed spacers and 5.8S rDNA, target sequences present in a high number of copies. The amplified products were digested with restriction enzymes in order to detect interspecific polymorphisms. Species‐specific restriction fragment length polymorphism patterns were determined for all five species. The use of PCR in conjunction with restriction enzymes provides a sensitive and efficient tool for use in distinguishing ectomycorrhizal species and monitoring inoculated seedlings or field mycorrhizal populations.