Open AccessThe detection of Gram‐negative bacterial mRNA from soil by RT‐PCR
Author(s) -
Mendum Tom A,
Sockett R.Elizabeth,
Hirsch Penny R
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13111.x
Subject(s) - reverse transcriptase , polymerase chain reaction , gram , biology , gene , microbiology and biotechnology , enzyme , bacteria , genetics , biochemistry
The method described extracts high quality RNA from a clay‐rich soil for which other published extraction protocols have failed. Transcript encoding the enzyme β‐glucuronidase could be reliably detected by reverse transcriptase polymerase chain reaction in soil seeded with a genetically modified Rhizobium leguminosarum containing the uidA gene.