Open AccessAllosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin‐C production of the fungus
Author(s) -
Sándor Erzsébet,
Pusztahelyi Tünde,
Karaffa Levente,
Karányi Zsolt,
Pócsi Imre,
Biró Sándor,
Szentirmai Attila,
Pócsi István
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13091.x
Subject(s) - chitinase , penicillium chrysogenum , cephalosporin c , acremonium , biochemistry , chemistry , enzyme , fragmentation (computing) , fungus , microbiology and biotechnology , cephalosporin , chitin , biology , antibiotics , ecology , botany , chitosan
The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin‐C production in Acremonium chrysogenum . In vitro inhibition of A. chrysogenum cell‐bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate.