Open AccessMorphological and 13 C‐nuclear magnetic resonance studies for polyhydroxyalkanoate biosynthesis in Pseudomonas sp. 61‐3
Author(s) -
Fukui Toshiaki,
Kato Mikiya,
Matsusaki Hiromi,
Iwata Tadahisa,
Doi Yoshiharu
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13089.x
Subject(s) - polyhydroxyalkanoates , pseudomonas , chemistry , biosynthesis , pseudomonadales , carbon fibers , pseudomonadaceae , nuclear chemistry , biochemistry , bacteria , stereochemistry , organic chemistry , materials science , biology , enzyme , genetics , composite number , composite material
Pseudomonas sp. 61‐3 is able to produce a blend of poly(3‐hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer [P(3HB‐ co ‐3HA)] consisting of 3‐hydroxyalkanoate units of 4–12 carbon atoms. In a cell accumulating polyhydroxyalkanoates upon glucose or alkanoic acids, both needle‐type and mushroom‐type structures were observed as PHA granules by freeze‐fracture electron microscopy, indicating that Pseudomonas sp. 61‐3 synthesized and stored both P(3HB) and P(3HB‐ co ‐3HA) granules simultaneously as separate granules in the same cell. 13 C‐NMR analysis of polyhydroxyalkanoates synthesized from 13 C‐labeled octanoate revealed that 3‐hydroxybutyrate units in the resultant polyhydroxyalkanoates were not only supplied via fatty acid β‐oxidation but also via dimerization of two acetyl‐CoA molecules in Pseudomonas sp. 61‐3. Approximately 26% of 3‐hydroxybutyrate units was found to be generated via dimerization of acetyl‐CoA when octanoate was fed as a carbon source.