Open AccessCo‐migration of RAPD‐PCR amplicons from Aeromonas hydrophila
Author(s) -
Oakey Helen J.,
Gibson Lewis F.,
George Anthony M.
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13064.x
Subject(s) - rapd , amplicon , aeromonas hydrophila , biology , polymerase chain reaction , genetics , typing , dna , dna profiling , genome , aeromonas , bacteria , microbiology and biotechnology , gene , genetic diversity , population , demography , sociology
Random amplified polymorphic DNA polymerase chain reaction (RAPD‐PCR) uses arbitrary primers and low stringency annealing conditions to amplify anonymous DNA fragments which are then depicted in agarose gels. RAPD‐PCR fingerprints have been used for typing and differentiation of bacteria and, increasingly, for the study of genetic relationships between strains and species of microorganisms, plants and animals. The analysis of such fingerprints is based upon the assumption that co‐migration of amplicons does not occur and that any given band contains a single amplicon. This report shows that co‐migration of fragments of nearly identical size, but different nucleotide sequences, occurs between different isolates and within single RAPD‐PCR bands from Aeromonas hydrophila . The possibility of the same phenomenon occurring for other prokaryotic or eukaryotic genomes argues for caution in the interpretation of RAPD‐PCR fingerprints.