Open AccessSingle step molecular characterization of morphologically similar black truffle species 1
Author(s) -
Rubini Andrea,
Paolocci Francesco,
Granetti Bruno,
Arcioni Sergio
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13060.x
Subject(s) - truffle , amplicon , internal transcribed spacer , biology , polymerase chain reaction , intraspecific competition , multiplex polymerase chain reaction , dna , spacer dna , genetics , botany , gene , zoology , phylogenetic tree
Species‐specific internal ITS primers that amplify polymerase chain reaction (PCR) products of different lengths were selected to distinguish the morphologically similar ectomycorrhizal fungi T. melanosporum , T. brumale and T. indicum by aligning their internal transcribed spacer sequences and taking into account any incidence of intraspecific variability. In multiplex PCR experiments, the species‐specific primers yielded the expected amplicons on template DNA isolated from the above mentioned species, while there was no amplification in PCR reactions carried out on fungal DNA from competing truffle species and host plants.