Open AccessTranscription of the groESL operon in Pseudomonas aeruginosa PAO1
Author(s) -
Fujita Masaya,
Amemura Akinori,
Aramaki Hironori
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13051.x
Subject(s) - groel , operon , transcription (linguistics) , promoter , biology , groes , sigma factor , microbiology and biotechnology , gene , genetics , heat shock protein , gene expression , escherichia coli , linguistics , philosophy
Northern hybridization and S1 nuclease mapping demonstrated that the groES and groEL genes in Pseudomonas aeruginosa were transcribed as a bicistronic mRNA of 2.2 kb. Two transcription start sites and a transcription termination site were mapped. Overlapping consensus sequences for σ 32 ‐ and σ 70 ‐dependent promoters were found in the upstream region of groES . Levels of groESL ‐specific mRNA were increased about 2‐fold upon heat shock. This response differs from the dramatic enhancement (more than 10‐fold) of groESL transcription after heat shock observed in other bacterial species.