Open AccessIsolation and characterization of the PEP‐phosphomutase and the phosphonopyruvate decarboxylase genes from the phosphinothricin tripeptide producer Streptomyces viridochromogenes Tü494
Author(s) -
Schwartz D.,
Recktenwald J.,
Pelzer S.,
Wohlleben W.
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13039.x
Subject(s) - tripeptide , gene cluster , biology , biosynthesis , mutant , gene , biochemistry , streptomyces , genetics , amino acid , bacteria
The previously isolated non‐phosphinothricin tripeptide producing Streptomyces viridochromogenes gene disruption mutant SP62/2 was used to identify and analyze genes encoding early steps of the phosphinothricin tripeptide biosynthesis. Cross‐feeding and bioconversion experiments between SP62/2 and known non‐phosphinothricin tripeptide producing mutants or presumptive phosphinothricin tripeptide precursors revealed that SP62/2 was blocked in step one or two of the phosphinothricin tripeptide biosynthesis. It was shown that the block in the biosynthesis is due to the integration of a temperature‐sensitive plasmid by illegitimate recombination into the phosphinothricin tripeptide biosynthetic gene cluster. The corresponding region was isolated from the wild‐type. A 2.7‐kb DNA fragment was analyzed comprising three ORFs ( ppm , ppd , orfX ) which are probably translationally coupled. The ppm gene encodes a protein which is similar to PEP‐phosphomutases and the deduced Ppd product shows similarity to the phosphonopyruvate decarboxylase from Streptomyces wedmorensis .