Open AccessCharacterization of the catecholate indicator strain S. typhimurium TA2700 as an ent fhuC double mutant
Author(s) -
Rabsch Wolfgang
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13029.x
Subject(s) - complementation , enterobactin , siderophore , escherichia coli , mutant , strain (injury) , mutation , biology , enterobacteriaceae , salmonella , gene , genetics , microbiology and biotechnology , phenotype , bacteria , chemistry , biochemistry , anatomy
The Salmonella typhimurium strain TA2700 is a good indicator strain for enterobactin and other catecholate siderophores. It is unable to use hydroxamate siderophores and α‐keto‐/α‐hydroxyacids and is resistant to albomycin, a sideromycin. By using a MudPQ mapping set the hydroxamate uptake mutation in TA2700 was located in the 2–5 Cs region. Using transduction with known markers, the mutation was mapped more precisely near 4 Cs. By complementation with different fhu genes from Escherichia coli , a complementation of the hydroxamate iron utilization phenotype was observed in fhuC ‐complemented TA2700 only. All fhuC ‐complemented strains were also sensitive to albomycin. A fhuC + transductant of S. typhimurium TA2700 was cross‐fed by α‐ketoisocaproic acid. By cotransduction with known and unknown markers near 13 Cs an ent mutation of S. typhimurium TA2700 was mapped.