Open AccessA heat‐inducible Bacillus subtilis bacteriophage Φ105 expression system for the production of the protective antigen of Bacillus anthracis
Author(s) -
Baillie L.W.J,
Moore P,
McBride B.W
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13024.x
Subject(s) - bacillus anthracis , bacillus subtilis , microbiology and biotechnology , immunogen , bacteriophage , antigen , biology , recombinant dna , bacillus (shape) , anthrax toxin , virology , bacteria , gene , fusion protein , escherichia coli , antibody , biochemistry , monoclonal antibody , immunology , genetics
The protective antigen of Bacillus anthracis is the major protective immunogen in the current human vaccine. A heat‐inducible protective antigen expression system was constructed based on a derivative of Bacillus subtilis phage Φ105. The recombinant protein produced by this system protected immunised animals against challenge with spores of B. anthracis . Gene instability and protease activity of the host strain contributed to the low level of recoverable protein in culture supernatant (∼2 mg l −1 ).