Open AccessCloning, characterization, and expression of the CIP2 gene induced under cadmium stress in Candida sp.
Author(s) -
Park Kill Soon,
Kwon Jung,
Choi SoonYong
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13016.x
Subject(s) - open reading frame , cadmium , gene , gene expression , cloning (programming) , messenger rna , oxidative stress , biology , chemistry , amino acid , microbiology and biotechnology , biochemistry , peptide sequence , organic chemistry , computer science , programming language
We isolated, sequenced, and expressed a cadmium‐induced gene, CIP2 , that specifically hybridizes to a mRNA of approximately 950 nucleotides. The CIP2 mRNA was barely present in normal Candida cells, but accumulated at higher levels in cadmium‐treated cells. Other heavy metals such as copper, mercury, lead, and manganese had no effect on the expression of the CIP2 gene. CIP2 contains an open reading frame encoding a protein of 203 amino acids. This gene was also activated by an oxidant, diamide. Our results suggest that CIP2 may play a crucial role in the specific cellular response to oxidative stress evolved by the cadmium treatment.