Open AccessA PCR‐based method to distinguish fungi of the rice sheath‐blight complex, Rhizoctonia solani, R. oryzae and R. oryzae‐sativae 1
Author(s) -
Johanson Andrea,
Turner Helen C,
McKay Gareth J,
Brown Averil E
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb13011.x
Subject(s) - rhizoctonia solani , rhizoctonia , sheath blight , biology , internal transcribed spacer , identification (biology) , xanthomonas oryzae pv. oryzae , botany , blight , microbiology and biotechnology , gene , oryza sativa , genetics , ribosomal rna
Identification of Rhizoctonia solani , R. oryzae and R. oryzae‐sativae , components of the rice sheath disease complex, is extremely difficult and often inaccurate and as a result may hinder the success of extensive breeding programmes throughout Asia. In this study, primers designed from unique regions within the rDNA internal transcribed spacers have been used to develop a rapid PCR‐based diagnostic test to provide an accurate identification of the species on rice. Tests on the specificity of the primers concerned showed that they provide the means for accurate identification of the Rhizoctonia species responsible for sheath diseases in rice.