Open AccessThe YGR194c ( XKS1 ) gene encodes the xylulokinase from the budding yeast Saccharomyces cerevisiae
Author(s) -
RodriguezPeña Jose Manuel,
Cid Victor J,
Arroyo Javier,
Nombela Cesar
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12993.x
Subject(s) - saccharomyces cerevisiae , gene , open reading frame , mutant , biology , genetics , plasmid , yeast , homology (biology) , gene product , gene expression , peptide sequence
We report the finding of a Saccharomyces cerevisiae gene necessary for growth in culture media with d ‐xylulose as the sole carbon source. This gene corresponds to the YGR194c open reading frame that we have previously described, and it is renamed now XKS1 . Data bank comparisons of the protein encoded by the XKS1 gene showed significant homology with different xylulokinases, indicating a possible role in xylulose phosphorylation. The wild‐type gene in a centromeric plasmid complemented defective growth of xks1 S. cerevisiae mutant strains in xylulose. By contrast, overexpression negatively influenced cell growth in this carbon source.