Open AccessThe leuX ‐encoded tRNA 5 Leu but not the pathogenicity islands I and II influence the survival of the uropathogenic Escherichia coli strain 536 in CD‐1 mouse bladder mucus in the stationary phase
Author(s) -
Dobrindt Ulrich,
Cohen Paul S,
Utley Maryjane,
Mühldorfer Inge,
Hacker Jörg
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12990.x
Subject(s) - escherichia coli , microbiology and biotechnology , strain (injury) , biology , enterobacteriaceae , pathogenicity , transfer rna , bacteria , genetics , pathogenicity island , gene , rna , anatomy
The uropathogenic Escherichia coli strain 536 carries two pathogenicity islands, each of which is associated with either of the tRNA genes sel C or leuX , respectively. Growth competition in CD‐1 mouse mucus between the wild‐type strain E. coli 536, its leuX mutant 536Δ102 and its mutant 536R3, lacking both pathogenicity islands but expressing a functional tRNA 5 Leu , revealed a major impact of leuX on E. coli survival in bladder mucus. The impaired survival in CD‐1 mouse mucus observed upon deletion of the leuX gene was abolished after complementation with the leuX gene. The survival of bacteria in bladder mucus was not influenced by the presence of pathogenicity islands I and II.