Open AccessCloning and characterization of the gene encoding the primary σ‐factor of Campylobacter jejuni
Author(s) -
Wösten Marc M.S.M,
Boeve Miranda,
Gaastra Wim,
Zeijst Bernard A.M
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12984.x
Subject(s) - campylobacter jejuni , cloning (programming) , gene , genetics , biology , campylobacter , microbiology and biotechnology , primary (astronomy) , molecular cloning , bacteria , computer science , peptide sequence , physics , programming language , astronomy
The rpoD gene encoding the primary σ‐factor of Campylobacter jejuni was amplified from genomic DNA with degenerate oligonucleotide primers. The complete gene encodes a polypeptide of 622 amino acids and has a deduced M r of 72.6 kDa. This polypeptide is 40% identical to the RpoD (σ 70 ) protein of Escherichia coli and has 66% identity with the Helicobacter pylori RpoD protein. A C. jejuni σ 70 promoter, not recognized by the E. coli σ 70 factor, could be activated in this bacterium in the presence of the cloned C. jejuni RpoD protein.