Open AccessThe detection of insertion sequences within the human pathogen Burkholderia pseudomallei which have been identified previously in Burkholderia cepacia
Author(s) -
Mack Kerri,
Titball Richard W
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12980.x
Subject(s) - burkholderia pseudomallei , burkholderia , biology , microbiology and biotechnology , pathogen , insertion sequence , melioidosis , polymerase chain reaction , homology (biology) , southern blot , bacteria , dna , genetics , virology , gene , genome , transposable element
Using primers designed from the nucleotide sequences of five insertion elements identified previously in Burkholderia cepacia , the presence of two insertion sequences (IS406 and IS407) was detected in chromosomal DNA isolated from strains of the human pathogen Burkholderia pseudomallei . The IS407 homologue was cloned from B. pseudomallei NCTC 4845 and nucleotide sequenced to confirm its identity and degree of homology with B. cepacia IS407. A PCR amplification product from B. pseudomallei NCTC 4845 DNA provided an IS407 probe which was used to determine, by Southern blotting, the number and location of copies of IS407 in ten strains of B. pseudomallei and four representatives from three of the five genomovars of B. cepacia .