Open AccessThe phylogenetic relationships of Chlorobium tepidum and Chloroflexus aurantiacus based upon their RecA sequences
Author(s) -
Gruber Tanja M,
Eisen Jonathan A,
Gish Kurt,
Bryant Donald A
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12978.x
Subject(s) - biology , phylogenetic tree , bacteria , phylogenetics , cytophaga , green sulfur bacteria , microbiology and biotechnology , genetics , gene , bacteriochlorophyll , pseudomonas , flavobacterium
Abstract Using RecA as the phylogenetic marker, the relationships of the green sulfur bacterium Chlorobium tepidum and the green non‐sulfur bacterium Chloroflexus aurantiacus to other eubacteria were investigated. The recA genes of the two organisms were cloned, and the resulting protein sequences aligned with 86 other eubacterial RecA sequences. Cb. tepidum was placed as the nearest relative to the Cytophaga / Flexibacter / Bacteroides group, a relationship supported by results obtained with several phylogenetic markers. Cf. aurantiacus was placed near Chlamydia trachomatis and the high‐GC Gram‐positives; however, this branching pattern was not strongly supported statistically by bootstrap analyses. Possible reasons for this ambiguity are discussed.