Open AccessAccumulation of poly‐β‐hydroxybutyrate by Rhodobacter sphaeroides on various carbon and nitrogen substrates
Author(s) -
Khatipov Emir,
Miyake Masato,
Miyake Jun,
Asada Yasuo
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12976.x
Subject(s) - rhodobacter sphaeroides , nitrogen , chemistry , ammonium , carbon fibers , rhodospirillaceae , biochemistry , nuclear chemistry , organic chemistry , photosynthesis , materials science , composite number , composite material
Combinations of various carbon and nitrogen substrates were used to study poly‐β‐hydroxybutyrate accumulation and H 2 evolution by Rhodobacter sphaeroides strain RV. The cells evolved hydrogen on lactate, pyruvate, or succinate under nitrogen‐deprived conditions and on lactate‐glutamate and pyruvate‐glutamate media. Poly‐β‐hydroxybutyrate content under nitrogen‐sufficient and nitrogen‐deprived conditions was highest on acetate (up to ∼40% of cell dry weight). Transfer of the cells from ammonium to ammonium‐free medium resulted in increases in the poly‐β‐hydroxybutyrate content on lactate and pyruvate of up to ∼15% and ∼20%, respectively. An increase in pH caused a decrease in H 2 production and an increase in poly‐β‐hydroxybutyrate accumulation on lactate under nitrogen‐deprived conditions.