Open AccessDisassociation of σ subunit from RNA polymerase of Xanthomonas oryzae pv. oryzae by phage Xp10 infection
Author(s) -
Lin ShanHua,
Liu JunSheng,
Yang BeiChang,
Kuo TsongTeh
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12972.x
Subject(s) - rna polymerase , specificity factor , polymerase , protein subunit , biology , microbiology and biotechnology , rna dependent rna polymerase , rna polymerase i , rna polymerase ii , rna , rna polymerase iii , antiserum , biochemistry , gene expression , enzyme , gene , antibody , promoter , genetics
The σ subunit of Xanthomonas oryzae pv. oryzae is disassociated from host RNA polymerase after phage Xp10 infection. To clarify the possible mechanism for this observation, σ subunit was purified and an antiserum against σ subunit was prepared. Immunoprecipitation of RNA polymerase by the anti‐core RNA polymerase antiserum, followed by immunoblotting with anti‐σ subunit antibody, revealed that σ subunit was lost from RNA polymerase within 10 minutes after Xp10 infection. Loss of σ subunit was not observed under other stress conditions including heat and cold stress, starvation and growth to stationary phase. Two‐dimensional immunoblotting analysis did not reveal any covalent modification of either σ subunit or RNA polymerase after Xp10 infection. These results suggest that separation of σ subunit from RNA polymerase may be due to competition with other binding factors.