Open AccessEdwardsiella tarda induces plasma membrane ruffles on infection of HEp‐2 cells
Author(s) -
Phillips Alan D,
Trabulsi Luiz R,
Dougan Gordon,
Frankel Gad
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12963.x
Subject(s) - edwardsiella tarda , microbiology and biotechnology , lysis , bacteria , cell , biology , pathogenesis , membrane , cell membrane , cytoskeleton , immunology , biochemistry , genetics
Abstract Interaction of two clinical Edwardsiella tarda isolates with HEp‐2 cells was investigated. By electron microscopy we observed at 1 h post infection that E. tarda induced formation of extensive plasma membrane projections resembling membrane ruffles. The ruffles did not coincide with adhering bacteria. Only few invading bacteria were seen. Vacuolated nuclear membrane was occasionally observed. Three hours post infection, E. tarda induced a contact‐dependent cell lysis, revealing the host cell cytoskeleton and nucleus. Only one of the E. tarda strains was seen residing within the host cell remains. The results indicate that E. tarda ‐induced membrane ruffles may involve a distinct mechanism of bacterial pathogenesis.