Open AccessGreen fluorescent protein as a novel marker and reporter system in Helicobacter sp.
Author(s) -
Josenhans Christine,
Friedrich Susanne,
Suerbaum Sebastian
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12957.x
Subject(s) - green fluorescent protein , biology , reporter gene , aequorea victoria , transposable element , plasmid , transposon mutagenesis , gene , mutagenesis , insertional mutagenesis , bioreporter , microbiology and biotechnology , promoter , mutant , genetics , gene expression
In order to be able to study gene regulation in single, live Helicobacter pylori bacteria in vitro or in contact with host cells, we established the green fluorescent protein gene gfp from Aequorea victoria as a reporter gene for use with Helicobacter species. We describe here the construction of genomic transcriptional fusions of the promoterless gfp gene with the flaA and flaB promoters of H. pylori . We have also constructed a Mini‐Tn 3 ‐km‐ gfp transposon to be used for shuttle transposon mutagenesis in H. pylori and H. mustelae . A marker strain with wild‐type phenotype, carrying multiple plasmid‐borne copies of gfp under the control of the H. pylori flaB promoter, was constructed for studies of bacterial distribution and transmission in animal models.