Open AccessMolecular cloning, expression, and characterization of dnaK in Streptococcus pneumoniae
Author(s) -
Kim SeungWhan,
Choi InHwa,
Kim SuNam,
Kim YongHwan,
Pyo SuhkNeung,
Rhee DongKwong
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12951.x
Subject(s) - immunogen , escherichia coli , biology , antiserum , microbiology and biotechnology , streptococcus pneumoniae , molecular cloning , cloning (programming) , staphylococcus aureus , peptide sequence , homology (biology) , bacteria , amino acid , gene , biochemistry , antibody , genetics , antibiotics , monoclonal antibody , computer science , programming language
DnaK is known to be highly conserved in all species and is a major immunogen in Streptococcus pneumoniae . To elucidate the role of dnaK in S. pneumoniae , dnaK was cloned in Escherichia coli using a homologous dnaK probe generated by PCR. The His‐tagged DnaK was overexpressed in soluble form and purified from E. coli . Alignment of the deduced DnaK amino acid sequence from nucleotide sequences of the cloned dnaK revealed high homology with DnaK analogs in E. coli (53%) and Staphylococcus aureus (73%). However, anti‐pneumococcal DnaK antiserum did not crossreact with DnaK analogs in E. coli , S. aureus and human cells suggesting that pneumococcal DnaK might be a good candidate as a vaccine.