Open AccessCalcium levels correlate with cell cycle phase and affect the level of the cyclin B transcript in Dictyostelium discoideum
Author(s) -
Azhar M,
Krefft M,
Saran S,
Weeks G,
Nanjundiah Vidyanand
Publication year - 1998
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1998.tb12948.x
Subject(s) - dictyostelium discoideum , microbiology and biotechnology , cell cycle , biology , dictyostelium , cyclin b , cyclin dependent kinase 1 , calcium , transcription (linguistics) , ionophore , cell , cyclin a , cyclin d1 , chemistry , gene , biochemistry , organic chemistry , linguistics , philosophy , membrane
In pre‐aggregation amoebae of Dictyostelium discoideum , phenotypic differences with respect to cellular Ca 2+ and cell cycle phases are known to bias post‐aggregative cell‐type choice. Using chlortetracycline fluorescence as an indicator, we found that cellular Ca 2+ is highest at the S phase of the cell cycle. Upon increasing the level of Ca 2+ with the help of the calcium ionophore A23187, there is a significant decrease in the cyclin B ( clb1 ) mRNA level; the cdc2 mRNA level shows a marginal decrease. These results suggest that the effect of Ca 2+ and the cell cycle on cell fate could be exerted at the level of transcription, or message stability, of specific genes.